burn wounds or receiving cancer chemotherapy (Nasreen et al., 2012). It is a major nosocomial pathogen, particularly dangerous to cystic fibrosis patients (Jayaraman et al., 2010). This organism is resistant to many antibacterial drugs. Multidrug-resistant P. aeruginosa phenotype is defined as resistant to one antimicrobial agent in three or more anti-pseudomonal anti-microbial classes (carbapenems, fluoroquinolones, penicillins, cephalosporins and aminoglycosides) (Ahmed et al., 2013).
The alarming rate of resistance in bacteria pathogens raises concern for the effectiveness of antibiotic therapy, (Nmema et al., 2013). The spread of multidrug-resistant pathogens is a real threat to public health and a major concern for infection control practitioners globally (Vishal et al., 2012). This spread has create a great concern, for the re-emergence of previously controlled diseases and a high frequency of opportunistic and chronic infection cases in developing countries more especially Nigeria( Vishal et al., 2012).
1.1 BACKGROUND OF STUDY
In 1882, Gessard first discovered Pseudomonas, a strictly aerobic, gram negative bacterium of low virulence. The organism is ubiquitous, with a predilection to most environments, primarily as waterborne and soil borne organisms.
Pseudomonas species have been found in soil, water, plant, and animals; pseudomonas aerugenosa colonization reportedly occurs in more than 50% of humans, and Pseudomonas is the most common Pseudomonal species. It is an opportunistic pathogen that causes nosocomial infections; it causes a serious life-threatening disease, these organisms’ exhibits innate resistances to many antibiotics and can develop new resistances after exposure to antimicrobial agents
Pseudomonas aeroginosa is one of the leading causes of hospital-acquire as well as community – acquired infection due to significant changes in microbial genetic ecology; as a result of indiscriminate use of antibiotics, the spread of multidrug resistance (MDR) is now a global problem. Its general resistances are due to a combination of factor. Several different epidemiological studies indicate that antibiotics resistances are increasing in clinical isolates.
Escherichia coli are the leading cause of urinary tract, ear, wound and other infections in humans. Increasing rates of antimicrobial resistance among E. coli is a growing concern worldwide.
1.2 STATEMENT OF THE RESEARCH PROBLEM
The research problem was to find out the antibiotics susceptibility pattern of Pseudomonas aeroginosa and Escherichia coli in tertiary health care inoculation in Clad, MacConkey, and Blood agar
1.3 AIM OF STUDY
To investigate the antibiotic susceptibility pattern of Gram -negative bacteria (Pseudomonas aerugenosa and Escherichia coli) isolated from clinical isolates of patients and to determine the age group commonly infected with bacteria.
1.4. OBJECTIVE OF STUDY
1. To determine antibiotics susceptibility pattern of clinical isolates of Pseudomonas aeroginosa and Escherichia coli collected from (Obafemi Awolowo University Teaching Hospital Complex,(OAUTHC) and Seventh Days Adventist (SDA) in ile-ife
2. The study will enlighten the general public and the effects of misuse, mismanagement and continuous use of antibiotics and further educate the general public on the importance of antibiotics sensitivity testing so as to avoid drugs resistance.